nicking response catalysed via the MobA89K and MobC89K proteins. The cleaved strand is then unwound by the helicase and sent to the 89K-encoded GI-variety T4SS machinery. The VirB1-89K component is thought to work as a lytic transglycosylase, locally opening the peptidoglycan with contributions from A few other issue(s) in the course of the assembl